Antigen

Antigen Class

TFs and others

Antigen

Xbp1

Cell type

Cell type Class

Liver

Cell type

Liver

MeSH Description

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Sample

source_name

LIVER

gender

male

strain

C57BL/6J

age

8-12 weeks of age

genotype

WT

time

CT40

tissue

liver

chip antibody

anti-XBP1s (biolegend 6105)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The DNA libraries were prepared at Univerisity of Pittsburgh and sequenced at Gene by Gene per standard protocols. DNA libraries were prepared with Ovation® Ultralow V2 DNA-Seq library preparation kit (NuGen) using 1ng input DNA. The size selection for libraries were performed using SPRIselect beads (Beckman Coulter) and purity of the libraries were analyzed using the High Sensitivity DNA chip on Bioanalyzer 2100 (Agilent). The prepared libraries pooled and sequenced using Nova-Seq 6000 SP flowcell (Illumina), generating ~30 million 75 bp single-end reads per samples.

Sequencing Platform

instrument_model

Illumina NovaSeq 6000

mm10

Number of total reads

64813100

Reads aligned (%)

96.4

Duplicates removed (%)

8.6

Number of peaks

444 (qval < 1E-05)

mm9

Number of total reads

64813100

Reads aligned (%)

96.3

Duplicates removed (%)

8.7

Number of peaks

438 (qval < 1E-05)